ifn i receptor subunit 1 ifnar1 blocking antibody  (Bio X Cell)

Journal: bioRxiv

Article Title: NLRP3 and AIM2 inflammasomes exacerbate the pathogenic Th17 cell response to eggs of the helminth Schistosoma mansoni

doi: 10.1101/2024.03.11.584371

Figure Lengend Snippet: (A) BMDCs from BL/6, NLRP3 -/- and AIM2 -/- BMDCs were cultured for 24h with 100 live (or no) eggs. IFNβ in supernatants was measured by ELISA. (B) BL/6, NLRP3 -/- and AIM2 -/- BMDCs were pretreated with IFN-I receptor blocking antibody (α-IFNAR1) (20μg/ml) or control IgG (20μg/ml) for 1h before culturing for 24h with 100 live (or no) eggs. IL-1β in supernatants was measured by ELISA. (C) The above mentioned BMDCs were co-cultured with T cells together with 100 live (or no) eggs. IL-17A in 72h supernatants were measured by ELISA. Bars represent the mean +/-SD cytokine levels of three biological replicates from one representative experiment of three with similar results. *p <0.05, **p <0.005, ***p <0.0005

Article Snippet: Diphenyleneiodonium, N-acetyl cysteine, Glybenclamide, Poly (dAdT), Red Blood Cell Lysing Buffer, and 2-mercaptoethanol were from Sigma, LPS was from Invivogen, Calcein AM from Invitrogen, GeneJuice from Novagen and the Caspase-1 inhibitor ZYVAD-FMK from APEx Bio and Nigericin was from Thermo Scientific, IFN-I receptor subunit 1 (IFNAR1) blocking antibody (MAR1-5A3) and IgG isotype control antibody (MOPC-21) from BioXCell.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Blocking Assay